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Retention of KOH-soluble fluoride on enamel and dentine under erosive conditions--A comparison of in vitro and in situ results.

Identifieur interne : 000410 ( Main/Exploration ); précédent : 000409; suivant : 000411

Retention of KOH-soluble fluoride on enamel and dentine under erosive conditions--A comparison of in vitro and in situ results.

Auteurs : Carolina Ganss [Allemagne] ; Nadine Schlueter ; Joachim Klimek

Source :

RBID : pubmed:17049481

Descripteurs français

English descriptors

Abstract

OBJECTIVES

Aim of the study was to investigate the stability of CaF(2)-like precipitates on enamel and dentine under neutral or acidic conditions and to compare in vitro and in situ results.

METHODS

Human enamel and dentine specimens were fluoridated (Elmex fluid, 5 min) and subjected to erosive demineralisation (Sprite light: 3/day each; 30 s in vitro, 2 min in situ) or stored under neutral conditions for 4 (in vitro) or 7 (in situ) days. KOH-soluble fluoride was determined using an ion selective electrode. Between the acid attacks, specimens were stored in remineralisation solution (in vitro) or retained in the oral cavity (four volunteers for enamel and dentine each).

RESULTS

Initially, high amounts of KOH-soluble fluoride were gained (between 77.9+/-12.3 and 96.0+/-46.4 microg/cm2). Under neutral conditions in vitro, a significant decrease on enamel (16.2+/-5.0 microg/cm2) and dentine (18.6+/-10.5 microg/cm2) was found, which was more severe under acidic conditions (6.3+/-3.0 and 5.1+/-2.1 microg/cm2, respectively). Under in situ conditions, KOH-soluble fluoride was strikingly stable on enamel under neutral (42.3+/-12.6 microg/cm2) as well as under acidic conditions (54.1+/-17.4 microg/cm2). On dentine, the dissolution kinetics of KOH-soluble fluoride was similar to the in vitro conditions, but the loss was less severe (45.3+/-12.9 microg/cm2 under neutral and 8.8+/-6.4 microg/cm2 under acidic conditions).

CONCLUSIONS

In vitro, more KOH-soluble fluoride was lost under erosive compared to neutral conditions. The intra-oral environment was considerably protective for CaF2-like precipitates especially on enamel.


DOI: 10.1016/j.archoralbio.2006.07.004
PubMed: 17049481


Affiliations:


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Le document en format XML

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<title xml:lang="en">Retention of KOH-soluble fluoride on enamel and dentine under erosive conditions--A comparison of in vitro and in situ results.</title>
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<name sortKey="Ganss, Carolina" sort="Ganss, Carolina" uniqKey="Ganss C" first="Carolina" last="Ganss">Carolina Ganss</name>
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<nlm:affiliation>Department of Conservative and Preventive Dentistry, Dental Clinic, Justus-Liebig-University, Giessen, Germany. carolina.ganss@dentist.med.uni-giessen.de</nlm:affiliation>
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<name sortKey="Schlueter, Nadine" sort="Schlueter, Nadine" uniqKey="Schlueter N" first="Nadine" last="Schlueter">Nadine Schlueter</name>
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<term>Amines (metabolism)</term>
<term>Dental Enamel (metabolism)</term>
<term>Dentin (metabolism)</term>
<term>Diamines (MeSH)</term>
<term>Fluoridation (methods)</term>
<term>Fluorides (metabolism)</term>
<term>Humans (MeSH)</term>
<term>Hydroxides (metabolism)</term>
<term>Molar (MeSH)</term>
<term>Potassium Compounds (metabolism)</term>
<term>Solubility (MeSH)</term>
<term>Tooth Erosion (metabolism)</term>
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<term>Amines (métabolisme)</term>
<term>Composés du potassium (métabolisme)</term>
<term>Dentine (métabolisme)</term>
<term>Diamines (MeSH)</term>
<term>Enrichissement en fluor (méthodes)</term>
<term>Fluorures (métabolisme)</term>
<term>Humains (MeSH)</term>
<term>Hydroxydes (métabolisme)</term>
<term>Molaire (MeSH)</term>
<term>Solubilité (MeSH)</term>
<term>Émail dentaire (métabolisme)</term>
<term>Érosion dentaire (métabolisme)</term>
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<term>Amines</term>
<term>Fluorides</term>
<term>Hydroxides</term>
<term>Potassium Compounds</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Dental Enamel</term>
<term>Dentin</term>
<term>Tooth Erosion</term>
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<term>Fluoridation</term>
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<term>Amines</term>
<term>Composés du potassium</term>
<term>Dentine</term>
<term>Fluorures</term>
<term>Hydroxydes</term>
<term>Émail dentaire</term>
<term>Érosion dentaire</term>
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<b>OBJECTIVES</b>
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<p>Aim of the study was to investigate the stability of CaF(2)-like precipitates on enamel and dentine under neutral or acidic conditions and to compare in vitro and in situ results.</p>
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<p>
<b>METHODS</b>
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<p>Human enamel and dentine specimens were fluoridated (Elmex fluid, 5 min) and subjected to erosive demineralisation (Sprite light: 3/day each; 30 s in vitro, 2 min in situ) or stored under neutral conditions for 4 (in vitro) or 7 (in situ) days. KOH-soluble fluoride was determined using an ion selective electrode. Between the acid attacks, specimens were stored in remineralisation solution (in vitro) or retained in the oral cavity (four volunteers for enamel and dentine each).</p>
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<b>RESULTS</b>
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<p>Initially, high amounts of KOH-soluble fluoride were gained (between 77.9+/-12.3 and 96.0+/-46.4 microg/cm2). Under neutral conditions in vitro, a significant decrease on enamel (16.2+/-5.0 microg/cm2) and dentine (18.6+/-10.5 microg/cm2) was found, which was more severe under acidic conditions (6.3+/-3.0 and 5.1+/-2.1 microg/cm2, respectively). Under in situ conditions, KOH-soluble fluoride was strikingly stable on enamel under neutral (42.3+/-12.6 microg/cm2) as well as under acidic conditions (54.1+/-17.4 microg/cm2). On dentine, the dissolution kinetics of KOH-soluble fluoride was similar to the in vitro conditions, but the loss was less severe (45.3+/-12.9 microg/cm2 under neutral and 8.8+/-6.4 microg/cm2 under acidic conditions).</p>
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<p>
<b>CONCLUSIONS</b>
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<p>In vitro, more KOH-soluble fluoride was lost under erosive compared to neutral conditions. The intra-oral environment was considerably protective for CaF2-like precipitates especially on enamel.</p>
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